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Updated: Mar 28, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Caryophyllene sesquiterpenoids from the endophytic fungus, Pestalotiopsis sp
Yi Liu1, Ming-Hua Yang1, Xiao-Bing Wang1
1State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Abstract:
Eight new caryophyllene sesquiterpenoids named pestaloporinates A-G (1-7) and 14-acetylhumulane (8) have been isolated from the solid cultures of an endophytic fungus Pestalotiopsis sp., which was obtained from the fresh stem bark of Melia azedarach Linn. Their structures as well as absolute configurations were determined by spectroscopic data, ECD experimentation, and single-crystal X-ray diffraction. Among all the isolates, compound 2 displayed potent inhibitory activity with IC50 value of 19.0 μM during the evaluation of nitric oxide (NO) inhibition in lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cells.
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