Related Experiment Video
Updated: Mar 28, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro
Colleen M Higgins1,2, Nicolas Bejerman3, Ming Li4,5
1Institute of Applied Ecology, School of Applied Sciences, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand. colleen.higgins@aut.ac.nz.
Abstract:
We report the first genome sequence of a Colocasia bobone disease-associated virus (CBDaV) derived from bobone-affected taro [Colocasia esculenta L. Schott] from Solomon Islands. The negative-strand RNA genome is 12,193 nt long, with six major open reading frames (ORFs) with the arrangement 3'-N-P-P3-M-G-L-5'. Typical of all rhabdoviruses, the 3' leader and 5' trailer sequences show complementarity to each other. Phylogenetic analysis indicated that CBDaV is a member of the genus Cytorhabdovirus, supporting previous reports of virus particles within the cytoplasm of bobone-infected taro cells. The availability of the CBDaV genome sequence now makes it possible to assess the role of this virus in bobone, and possibly alomae disease of taro and confirm that this sequence is that of Colocasia bobone disease virus (CBDV).
Related Concept Videos
Subviral Agents
Viruses with RNA Genomes
Cytomegalovirus Disease
Bacterial Gastroenteritis
Viruses of Archaea
Human Virome

