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MicroRNA-663 suppresses cell invasion and migration by targeting transforming growth factor beta 1 in papillary

Zhihong Wang1, Hao Zhang2, Ping Zhang1

  • 1Department of Thyroid Surgery, The First Hospital of China Medical University, Shenyang, 110001, People's Republic of China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|December 22, 2015
PubMed

Insights

MicroRNA-663 (miR-663) is downregulated in papillary thyroid carcinoma (PTC). Upregulating miR-663 suppresses PTC cell invasion and migration by targeting transforming growth factor beta 1 (TGFβ1).

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • MicroRNA-663 (miR-663) exhibits dual roles in various cancers.
  • Downregulation of miR-663 was observed in papillary thyroid carcinoma (PTC) via microarray analysis.
  • The specific function of miR-663 in PTC cell invasion and migration remained unclear.

Purpose of the Study:

  • To investigate the role of miR-663 in papillary thyroid carcinoma (PTC).
  • To determine the effect of miR-663 on PTC cell invasion and migration.
  • To elucidate the underlying molecular mechanism of miR-663 in PTC.

Main Methods:

  • Quantitative real-time PCR to measure miR-663 expression in 91 PTC tissues and cell lines.
  • Transwell and wound healing assays to assess PTC cell invasion and migration.
  • Reporter assays and rescue experiments to identify miR-663 targets and mechanisms.

Main Results:

  • miR-663 expression was significantly inhibited in PTC tissues and cell lines.
  • miR-663 upregulation suppressed PTC cell invasion and migration.
  • Transforming growth factor beta 1 (TGFβ1) was identified as a direct target of miR-663.
  • miR-663 regulates epithelial-mesenchymal transition (EMT) markers and matrix metalloproteinases (MMPs) via TGFβ1.

Conclusions:

  • miR-663 acts as a tumor suppressor in PTC.
  • miR-663 inhibits PTC cell invasion and migration by targeting TGFβ1.
  • Targeting TGFβ1 by miR-663 regulates EMT progression in PTC.
Keywords:
InvasionMigrationPapillary thyroid carcinomaTGFβ1miR-663

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