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Published on: August 10, 2018
Expression of CD105 on expanded mesenchymal stem cells does not predict their chondrogenic potential
M A Cleary1, R Narcisi2, K Focke2
1School of Veterinary Medicine, Veterinary Science Centre, University College Dublin, Belfield, Dublin 4, Ireland; Department of Orthopaedics, Erasmus MC, University Medical Centre, 3015 CN Rotterdam, The Netherlands.
CD105 expression does not identify chondrogenic bone marrow-derived mesenchymal stem cells (BMSCs). This study found no correlation between CD105 levels and chondrogenic potential in BMSCs, suggesting it is not a reliable marker for isolating these cells.
Area of Science:
- Stem cell biology
- Tissue engineering
- Cellular differentiation
Background:
- Bone marrow-derived mesenchymal stem cells (BMSCs) are a heterogeneous population.
- Individual BMSCs exhibit varying capacities for chondrogenesis (cartilage formation).
- Identifying specific markers to isolate chondrogenic subpopulations is crucial for regenerative medicine.
Purpose of the Study:
- To investigate if CD105 expression can be used to isolate a chondrogenic subpopulation of BMSCs.
- To determine the relationship between CD105 expression levels and chondrogenic potential in expanded BMSC populations.
Main Methods:
- BMSCs were isolated from patients and expanded in culture.
- Flow cytometry (FACS) was used to analyze CD105 expression and sort cells (CD105(+)/CD105(-), CD105(Bright)/CD105(Dim)).
- Chondrogenic differentiation was assessed by glycosaminoglycan (GAG) and collagen type II content.
Main Results:
- Expanded BMSC populations were predominantly CD105(+), with no correlation between CD105 percentage and chondrogenic potential.
- Chondrogenic capacity decreased with culture, while CD105 expression remained stable.
- No superior chondrogenic subpopulation was identified based on CD105 expression levels (Bright vs. Dim).
Conclusions:
- CD105 expression on cultured BMSCs does not correlate with chondroprogenitor phenotype.
- CD105 is not a reliable marker for isolating chondrogenic subpopulations from heterogeneous BMSC cultures.
- Further research is needed to identify effective markers for chondroprogenitor isolation.
