Related Experiment Video
Updated: Mar 28, 2026
06:52
Novel Assay for Cold Nociception in Drosophila Larvae
Published on: April 3, 2017
8.2K
Comparative transcriptome analysis revealed the genotype specific cold response mechanism in tobacco
Risheng Hu1, Xianxin Zhu2, Shipeng Xiang3
1Central South Agricultural Experiment Station of China Tobacco, Changsha, 410004, China.
Biochemical and Biophysical Research Communications
|December 23, 2015
Summary
Cold stress significantly impacts tobacco crops. This study identified key cold-responsive genes and pathways, including the circadian clock, offering valuable insights for developing cold-tolerant varieties.
Area of Science:
- Plant Science
- Genomics
- Environmental Stress Biology
Background:
- Cold stress poses a significant threat to plant growth, development, productivity, and quality.
- Understanding the genetic mechanisms underlying plant responses to cold is crucial for agricultural sustainability.
Purpose of the Study:
- To conduct a comparative genome-wide transcriptome analysis of cold-tolerant (NC567) and cold-sensitive (Taiyan8) tobacco cultivars under cold stress.
- To identify and functionally characterize cold-responsive (COR) genes and pathways involved in tobacco's cold stress response.
Main Methods:
- RNA-sequencing (RNA-seq) technology was employed for transcriptome analysis.
- Comparative analysis of gene expression profiles between two tobacco cultivars under cold conditions.
- Functional classification and pathway enrichment analysis of identified COR genes.
Main Results:
- Genome-wide transcriptome analysis identified a substantial number of unigenes in both tobacco cultivars.
- Cold-responsive genes involved in cell wall metabolism, transcription factors, the ubiquitin-proteasome system (UPS), and signaling were over-represented.
- COR genes were specifically induced in the cold-tolerant NC567 cultivar, with significant enrichment in the plant circadian clock pathway.
Conclusions:
- Highly induced expression of COR genes and the conserved mechanism of circadian clock-related genes play positive roles in tobacco's response to cold stress.
- The study provides valuable candidate genes for improving crop tolerance to cold stress.
- Comparative transcriptomics offers a powerful approach to dissecting plant stress responses.
Related Concept Videos
02:45Responses to Heat and Cold Stress
15.7K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
15.7K
02:27Background and Environment Affect Phenotype
8.1K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
8.1K
01:25Cell Signaling in Plants
7.0K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
7.0K
01:10Transcription
158.8K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
158.8K
02:22Introduction to Plant Diversity
50.4K
From Water to Land
50.4K
02:04Regulation of Transpiration by Stomata
32.2K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
32.2K