Related Experiment Video
Updated: Mar 28, 2026
Rodent Model of Intestinal Ischemia-Reperfusion Injury via Occlusion of the Superior Mesenteric Artery
Published on: October 20, 2023
A New Approach Using Manganese-Enhanced MRI to Diagnose Acute Mesenteric Ischemia in a Rabbit Model: Initial
Da-Wei Zhao1, Cheng Cheng1, Lian-Qin Kuang1
1Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Purpose:
Manganese-enhanced MRI (MEMRI) has been applied to a wide range of biological and disease research. The purpose of the study was to use MEMRI to diagnose the acute mesenteric ischemia (AMI).
Methods:
The institutional experimental animal ethics committee approved this study. To optimize the dose of Mn(2+) infusion, a dose-dependent curve was obtained using Mn(2+)-enhanced T 1 map MRI by an intravenous infusion 2.5-20 nmol/g body weight (BW) of 50 nmol/L MnCl2. The eighteen animals were divided into control, sham-operated, and AMI groups. AMI models were performed by ligating the superior mesenteric artery (SMA). T 1 values were measured on T 1 maps in regions of the small intestinal wall and relaxation rate (ΔR 1) was calculated.
Results:
A nonlinear relationship between infused MnCl2 solution dose and increase in small intestinal wall ΔR 1 was observed. Control animal exhibited significant Mn(2+) clearance over time at the dose of 15 nmol/g BW. In the AMI model, ΔR 1 values (0.95 ± 0.13) in the small intestinal wall were significantly lower than in control group (2.05 ± 0.19) after Mn(2+) infusion (P < 0.01).
Conclusion:
The data suggest that MEMRI shows potential as a diagnostic technique that is directly sensitive to the poor or absent perfusion in AMI.
Insights
Manganese-enhanced MRI (MEMRI) shows promise for diagnosing acute mesenteric ischemia (AMI). This technique detects reduced manganese uptake in the intestinal wall, indicating poor perfusion in AMI patients.
Area of Science:
- Biomedical imaging
- Diagnostic techniques
- Medical research
Background:
- Manganese-enhanced MRI (MEMRI) is a versatile tool in biological and disease research.
- Acute mesenteric ischemia (AMI) is a critical condition requiring timely diagnosis.
Purpose of the Study:
- To evaluate the efficacy of MEMRI as a diagnostic method for acute mesenteric ischemia (AMI).
- To assess the sensitivity of MEMRI to perfusion deficits in the small intestinal wall.
Main Methods:
- Optimized Mn(2+) infusion dose using a dose-dependent curve with T1-map MRI.
- Established AMI models in animals by ligating the superior mesenteric artery (SMA).
- Measured T1 values and calculated relaxation rate (ΔR1) in the small intestinal wall.
Main Results:
- A nonlinear relationship was observed between MnCl2 dose and small intestinal wall ΔR1.
- Control animals showed significant Mn(2+) clearance over time.
- AMI models exhibited significantly lower ΔR1 values in the small intestinal wall compared to controls (0.95 ± 0.13 vs. 2.05 ± 0.19, P < 0.01).
Conclusions:
- MEMRI demonstrates potential as a diagnostic technique for AMI.
- The technique is directly sensitive to perfusion abnormalities characteristic of AMI.
