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Updated: Mar 28, 2026
Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations
Published on: December 16, 2017
Living cell imaging and Rac1-GTP levels of CXCL12-treated migrating neural progenitor cells in stripe assay
Min Zhang1, Aihong Song1, Siqiang Lai1
1Center for Translational Neurodegeneration and Regenerative Therapy, Shanghai Tenth People's Hospital affiliated to Tongji University School of Medicine, Shanghai, China.
Abstract:
This data article contains three figures and three videos related to the research article entitled "Applications of Stripe Assay in the Study of CXCL12-mediated Neural Progenitor Cell Migration and Polarization" Zhang et al. (2015) [1], which uses stripe assay to study mouse neural progenitor cell (NPC) migration and polarization. The current article describes the neurosphere method used to culture NPCs. NPCs in neurospheres and monolayer were characterized using immunocytochemistry method with antibodies against two classic NPC markers: nestin and SOX2. The article also describes method to obtain sufficient protein lysates from NPCs in the stripe assay. When protein lysates were subjected to Rac1 affinity precipitation, Rac1-GTP was detected in the pull-down samples. In addition, the articles provides live cell imaging data to better understand CXCL12-mediated cellular migration and polarization.
