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Magnetic Resonance Imaging

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...

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Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

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Published on: September 20, 2015

A CatalyCEST MRI Contrast Agent that Can Simultaneously Detect Two Enzyme Activities.

Gabriela Fernández-Cuervo¹, Sanhita Sinharay², Mark D Pagel³

¹Department of Pharmaceutical Sciences, University of Arizona, 1515 N. Campbell Ave., Tucson, AZ, 85724, USA.

Chembiochem : a European Journal of Chemical Biology

|December 24, 2015

Summary

This study introduces a novel diamagnetic chemical exchange saturation transfer (CEST) MRI contrast agent for simultaneous detection of two enzyme activities. This platform technology enhances diagnostic specificity by requiring dual enzyme activation for MRI signal generation.

Keywords:

enzyme catalysis exchange interactions hydrogen bonds imaging agents

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Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Published on: December 4, 2016

Area of Science:

Biomedical Imaging
Molecular Imaging
Contrast Agent Development

Background:

Simultaneous detection of multiple enzyme activities can enhance disease diagnostic specificity.
Current molecular imaging techniques may lack the ability to detect multiple biomarkers concurrently.

Purpose of the Study:

To synthesize and characterize a novel diamagnetic chemical exchange saturation transfer (CEST) MRI contrast agent.
To develop a platform technology capable of simultaneously detecting two enzyme activities for improved disease diagnosis.

Main Methods:

Synthesis and characterization of a diamagnetic CEST MRI contrast agent.
Enzyme-mediated cleavage of agent ligands by sulfatase and esterase enzymes.
Detection of released salicylic acid using CEST MRI.

Main Results:

The developed CEST agent simultaneously detects sulfatase and esterase activities.
Both enzymes are required to activate the agent and produce CEST MRI contrast.
The agent demonstrated stability in the absence of enzyme treatment.

Conclusions:

The diamagnetic CEST MRI contrast agent functions as a platform technology with a modular design.
This agent can be potentially adapted to detect other combinations of enzyme activities.
The developed agent expands the available contrast agents for molecular imaging applications.