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High Content Imaging (HCI) on Miniaturized Three-Dimensional (3D) Cell Cultures
1Department of Chemical & Biomedical Engineering, Cleveland State University, 1960 East 24th Street Cleveland, Ohio, OH 44115-2214, USA. p.joshi18@vikes.csuohio.edu.
Biosensors
|December 24, 2015
Summary
High content imaging (HCI) assays are crucial for drug screening. This review highlights miniaturized 3D cell culture platforms that enable high-throughput screening with HCI, improving drug toxicity and efficacy predictions.
Area of Science:
- Biotechnology
- Cell Biology
- Pharmacology
Background:
- High content imaging (HCI) is a multiplexed cell staining assay vital for understanding biological functions and drug mechanisms.
- Conventional HCI on 2D cell cultures limits in vivo predictability for drug toxicity and efficacy.
- Three-dimensional (3D) cell cultures mimic in vivo environments but traditionally lack high-throughput screening (HTS) capabilities.
Purpose of the Study:
- To review miniaturized 3D cell culture platforms compatible with current HCI technology.
- To address the need for HTS-amenable 3D cell culture models in drug discovery.
Main Methods:
- Literature review focusing on miniaturized 3D cell culture platforms.
- Analysis of platform compatibility with high content imaging (HCI) technology.
Main Results:
- Miniaturized 3D cell culture platforms offer a solution to bridge the gap between 3D cell culture and HTS.
- These platforms enhance the predictive power of drug screening by better mimicking in vivo conditions.
Conclusions:
- Miniaturized 3D cell culture platforms are essential for advancing HCI in drug discovery.
- Integrating these platforms with HCI can significantly improve the efficiency and accuracy of toxicity and efficacy screening.
