Expression of Na,K-ATPase and H,K-ATPase Isoforms with the Baculovirus Expression System
Jan B Koenderink1, Herman G P Swarts2
1Pharmacology/Toxicology 149, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, 9101, 6500 HB, Nijmegen, The Netherlands. Jan.Koenderink@radboudumc.nl.
Abstract:
P-type ATPases can be expressed in several cell systems. The baculovirus expressions system uses an insect virus to enter and express proteins in Sf9 insect cells. This expression system is a lytic system in which the cells will die a few days after viral infection. Subsequently, the expressed proteins can be isolated. Insect cells are a perfect system to study P-type ATPases as they have little or no endogenous Na,K-ATPase activity and other ATPase activities can be inhibited easily. Here we describe in detail the expression and isolation of Na,K-ATPase and H,K-ATPase isoforms with the baculovirus expression system.
