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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
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Zebrafish Whole-Mount In Situ Hybridization Followed by Sectioning
Canan Doganli1, Jens Randel Nyengaard2, Karin Lykke-Hartmann3,4
1Smith Cardiovascular Research Institute, University of California, San Francisco, CA, 94158-9001, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 24, 2015
Abstract:
In situ hybridization is a powerful technique used for locating specific nucleic acid targets within morphologically preserved tissues and cell preparations. A labeled RNA or DNA probe hybridizes to its complementary mRNA or DNA sequence within a sample. Here, we describe RNA in situ hybridization protocol for whole-mount zebrafish embryos.
