Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Major Depressive Disorder From a Brain-Body Perspective: Reproducible Central Cardiac Interoception Deficits and Peripheral Autonomic Dysfunctions Dissociate.

Biological psychiatry·2026
Same author

Multiplexed electrochemical biosensors: system-level design frameworks, signal decoding strategies and translational perspectives for food safety.

Food research international (Ottawa, Ont.)·2026
Same author

Label-free electrochemical split-aptamer aptasensor with signal amplification via flower-like gold nanostructures for ultrasensitive detection of kanamycin in water.

Talanta·2026
Same author

CIRCLE-F/V: a dual-mode CRISPR-Cas13a cascade biosensor for ultrasensitive and visual detection of low-abundance EGFR mutations.

Journal of biological engineering·2026
Same author

Recent advances in electrochemical-based CRISPR/Cas biosensing for nucleic acid and non-nucleic acid pathogenic microorganism detection.

Food research international (Ottawa, Ont.)·2026
Same author

Exploration and validation of biomarkers for Alzheimer's disease based on GEO database.

IBRO neuroscience reports·2026

Related Experiment Video

Updated: Mar 28, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K

Reproducible E. coli detection based on label-free SERS and mapping.

Danting Yang1, Haibo Zhou2, Christoph Haisch3

  • 1College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

Talanta
|December 24, 2015
PubMed
Summary

This study presents a novel label-free surface-enhanced Raman scattering (SERS) method for rapid and reproducible detection of Escherichia coli bacteria. The optimized SERS technique achieves high sensitivity, enabling bacterial strain discrimination and detection at low concentrations.

Keywords:
Discriminant analysisE. coliLabel-freeSERSSingle bacterium cell

More Related Videos

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

5.0K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.6K

Related Experiment Videos

Last Updated: Mar 28, 2026

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging
06:19

Author Spotlight: Advancing SERS Technology: Au@Carbon Dot Nanoprobes for Label-Free Analysis and Imaging

Published on: June 9, 2023

2.4K
Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
10:59

Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

Published on: May 28, 2021

5.0K
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

21.6K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microbiology

Background:

  • Surface-enhanced Raman scattering (SERS) is a powerful technique for biosensing.
  • Achieving sensitive, reproducible, and label-free bacterial detection remains a challenge.

Purpose of the Study:

  • To develop a rapid, label-free SERS method for detecting and discriminating Escherichia coli (E. coli).
  • To optimize incubation conditions for enhanced SERS signal and reproducibility.

Main Methods:

  • Utilized a label-free SERS approach with silver colloid incubation for E. coli detection.
  • Optimized parameters including shaking speed, time, and temperature.
  • Employed discriminant analysis for strain discrimination and SERS mapping for sensitivity analysis.

Main Results:

  • Achieved a Raman signal enhancement of 1.8×10(4) cps with high reproducibility.
  • Successfully discriminated three strains of E. coli (DSM 1116/498/5695).
  • Detected E. coli at concentrations as low as 1×10(5) cell/mL via SERS mapping.

Conclusions:

  • The developed label-free SERS method offers superior sensitivity and reproducibility compared to existing simple-mixing techniques.
  • This approach provides a promising platform for developing advanced SERS-based biosensors for bacterial analysis.