Related Experiment Video
Updated: Mar 28, 2026

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
Improving 3'-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking
Tzi-Yuan Wang1, Yi-Hsuan Tsai2, I-Zen Yu2
1Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan, Republic of China.
Abstract:
3'-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3'-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3'-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3'- hydroxygenistein, indicating a high potential for industrial-scale 3'-hydroxygenistein production.
Related Concept Videos
Bioreactor Controls-III
Upstream Processing
Bioreactor Controls-II
Peroxisomes

