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Thermoresponsive amperometric glucose biosensor.

Piyanut Pinyou1, Adrian Ruff1, Sascha Pöller1

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This study presents a novel thermoresponsive biosensor for glucose detection. The biosensor utilizes a temperature-triggered polymer to switch its activity on and off, enabling precise control without heating the sample.

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Area of Science:

  • Electrochemistry
  • Materials Science
  • Biotechnology

Background:

  • Biosensors are crucial for detecting biomolecules like glucose.
  • Controlling biosensor activity often requires external manipulation of the sample or sensor.
  • Thermoresponsive polymers offer potential for on-demand control of biomolecule interactions.

Purpose of the Study:

  • To fabricate a thermoresponsive biosensor for amperometric glucose detection.
  • To develop a system for switching biosensor activity using temperature.
  • To investigate the role of a thermoresponsive polymer in controlling biosensor function.

Main Methods:

  • Fabrication of screen printed electrodes with gold working electrodes.
  • Modification of electrodes via electrochemically induced codeposition of a thermoresponsive copolymer (Polymer I) and a redox-polymer (Polymer II) functionalized with osmium complexes.
  • Immobilization of pyrroloquinoline quinone-soluble glucose dehydrogenase as the biological recognition element.
  • Amperometric detection of glucose with temperature-dependent measurements.

Main Results:

  • The biosensor demonstrated successful amperometric detection of glucose.
  • A thermoresponsive copolymer (Polymer I) with a lower critical solution temperature around 28°C was employed as a temperature-triggered immobilization matrix.
  • Biosensor activity was significantly reduced above the phase transition temperature of Polymer I, indicating an on/off switching capability.
  • Local temperature modulation at the electrode-bioactive layer interface allowed for switching the biosensor's state.

Conclusions:

  • A novel thermoresponsive biosensor for glucose detection was successfully fabricated.
  • The biosensor exhibits controllable on/off switching of activity via local temperature modulation.
  • This approach allows for precise control of biosensor function without heating the bulk analyte solution.