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Mast cell chymase in experimentally induced psoriasis
Mireille-Maria Suttle1, Ilkka T Harvima1
1Department of Dermatology, University of Eastern Finland and Kuopio University Hospital, Kuopio, Finland.
The Journal of Dermatology
|December 26, 2015
Summary
Mast cell chymase activity influences psoriasis development. Low chymase activity may promote disease, while high activity appears to control it, suggesting a dual role in psoriatic immunopathogenesis.
Area of Science:
- Immunology
- Dermatology
- Biochemistry
Background:
- Mast cell chymase exhibits dual pro-inflammatory and immunosuppressive roles in psoriasis.
- The specific activity level of chymase dictates its functional outcome in the disease.
Purpose of the Study:
- To investigate mast cell chymase activity and immunoreactivity during the Köbner reaction in psoriasis.
- To determine the effect of recombinant human chymase and LAD2 mast cells on psoriatic immune cells.
Main Methods:
- Studied mast cell chymase activity (Chyact) and immunoreactivity (Chyprot) in psoriasis biopsies post-tape-stripping.
- Assessed the impact of recombinant human chymase (rh-chymase) and LAD2 mast cells on (3H)-thymidine uptake in psoriatic peripheral blood mononuclear cells (PBMC) and T cells.
Main Results:
- The chymase activity/immunoreactivity ratio was higher in Köbner-negative than Köbner-positive biopsies.
- Low concentrations of rh-chymase stimulated PBMC and T cells, while high concentrations (5 μg/mL) induced inhibition.
- LAD2 mast cells inhibited PBMC without SBTI but stimulated PBMC in its presence.
Conclusions:
- Psoriatic immunopathogenesis may be exacerbated by low mast cell chymase activity.
- High mast cell chymase activity appears to exert a regulatory or inhibitory effect on psoriatic immune responses.
