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Human mesenchymal stem cell behavior on segmented polyurethanes prepared with biologically active chain extenders.
Taylor E Kavanaugh1, Amy Y Clark2, Lerma H Chan-Chan3
1Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA, 30332, USA.
Journal of Materials Science. Materials in Medicine
|December 26, 2015
Summary
Segmented polyurethanes (SPUs) were modified with osteogenic compounds for tissue engineering. Only SPUs with beta-glycerol phosphate supported stem cell growth and bone regeneration, showing potential for regenerative medicine.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Polymer Chemistry
Background:
- Elastomeric, bioresorbable, and biocompatible segmented polyurethanes (SPUs) are crucial for tissue engineering scaffolds.
- A significant limitation is the restricted incorporation of osteoinductive molecules into SPUs.
- There is a need for mechanically tunable scaffolds for diverse tissue regeneration.
Purpose of the Study:
- To synthesize novel segmented polyurethanes (SPUs) incorporating osteogenic compounds.
- To evaluate the cytocompatibility and osteogenic potential of these modified SPUs.
- To explore their suitability for bone tissue regeneration applications.
Main Methods:
- SPUs synthesized using poly(ε-caprolactone)diol and 4,4'-methylene bis(cyclohexyl isocyanate) with ascorbic acid, L-glutamine, β-glycerol phosphate, or dexamethasone as chain extenders.
- Characterization via FTIR, DSC, DMA, XRD, and mechanical testing.
- In vitro cytocompatibility and osteogenic differentiation assays using human mesenchymal stem cells.
Main Results:
- FTIR confirmed urethane and urea linkages. Polymers exhibited semi-crystalline nature and high deformation.
- Only SPUs incorporating β-glycerol phosphate supported human mesenchymal stem cell adhesion, growth, and osteogenic differentiation.
- Other modified SPUs failed to support cell growth or osteogenic differentiation.
Conclusions:
- Modification of SPUs with specific osteogenic compounds can yield cytocompatible polymers.
- SPUs containing β-glycerol phosphate show promise for bone tissue engineering.
- This research opens avenues for developing advanced biomaterials in regenerative medicine.
