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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
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The decrease of cyclin B2 expression inhibits invasion and metastasis of bladder cancer.

Cheng-yong Lei1, Wei Wang2, Yong-tong Zhu3

  • 1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China.

Urologic Oncology
|December 27, 2015
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Cyclin B2 is overexpressed in bladder cancer. Reducing cyclin B2 levels inhibits cancer cell invasion and metastasis, improving survival in mice.

Keywords:
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Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Cyclin B2 is frequently overexpressed in various malignant tumors.
  • Its specific role in bladder cancer pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the role of cyclin B2 in bladder cancer.
  • To determine the effect of cyclin B2 down-regulation on cancer cell behavior.

Main Methods:

  • Immunohistochemistry, Western blotting, and qRT-PCR were used to assess cyclin B2 expression in bladder tissues and cell lines.
  • Bladder cancer cells with reduced cyclin B2 levels were generated for in vitro and in vivo experiments.

Main Results:

  • Cyclin B2 protein and mRNA levels were significantly higher in bladder cancer tissues and cells compared to normal controls.
  • Down-regulation of cyclin B2 inhibited cell invasion and metastasis in vitro and in vivo.
  • Reduced cyclin B2 expression did not significantly affect cell growth or cell cycling.

Conclusions:

  • Cyclin B2 is overexpressed in bladder cancer.
  • Targeting cyclin B2 can inhibit bladder cancer cell invasion and metastasis.
  • Down-regulation of cyclin B2 may represent a therapeutic strategy to improve patient survival.