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Related Experiment Videos

Nuclear envelope disassembly and nuclear lamina depolymerization during germinal vesicle breakdown in starfish.

S A Stricker1, G Schatten

  • 1Integrated Microscopy Resource for Biomedical Research, University of Wisconsin, Madison 53706.

Developmental Biology
|September 1, 1989
PubMed
Summary

Starfish oocyte maturation involves germinal vesicle breakdown (GVBD). The nuclear envelope disassembles before the nuclear lamina fully depolymerizes, preceding GVBD completion.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Marine Biology

Background:

  • Germinal vesicle breakdown (GVBD) is a critical event in oocyte maturation.
  • The precise temporal sequence of nuclear envelope and nuclear lamina disassembly during GVBD is not fully understood.

Purpose of the Study:

  • To elucidate the sequential events of nuclear envelope and nuclear lamina disassembly during starfish oocyte maturation.
  • To investigate the role of protein phosphorylation in GVBD.

Main Methods:

  • Correlative ultrastructural analysis
  • Immunolabeling techniques
  • Light microscopy
  • Immunofluorescence studies

Main Results:

Related Experiment Videos

  • In Pisaster ochraceus oocytes, nuclear envelope disassembly precedes nuclear lamina depolymerization by 10-20 minutes.
  • Completion of GVBD occurs approximately 15 minutes after complete nuclear lamina depolymerization.
  • Increased nuclear protein phosphorylation precedes structural changes in the nuclear envelope and lamina.

    • Conclusions:

    • Nuclear envelope disassembly is an early event during GVBD, occurring before complete nuclear lamina depolymerization.
    • Nuclear lamina depolymerization is a rate-limiting step for the completion of GVBD.
    • Protein phosphorylation plays a significant role in initiating the structural reorganization during GVBD.