Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

InNMR: Direct <i>In Situ</i> Studies of Transport Phenomena Enabled by an Innovative NMR-Tube Insert.

Analytical chemistry·2026
Same author

Alginate-Amphotericin B bifunctional conjugates have improved solubility and reduced toxicity.

Carbohydrate polymers·2026
Same author

Physical-mechanical properties of fucoidan-alginate composite hydrogels.

Carbohydrate polymers·2026
Same author

Dissecting a two-domain alginate lyase of family PL6 reveals a mechanistic basis for substrate specificity and enzyme activity.

The Journal of biological chemistry·2026
Same author

An Accessible Platform to Quantify Oxygen Diffusion in Cell-Laden Hydrogels and Its Application to Alginate-Immobilized Pancreatic Beta Cells.

Biotechnology and bioengineering·2025
Same author

Unaltered 3'-sialyllactose and 6'-sialyllactose concentrations in human milk acutely after endurance exercise: a randomized crossover trial.

Frontiers in nutrition·2025

Related Experiment Video

Updated: Mar 28, 2026

Generation of Alginate Microspheres for Biomedical Applications
10:33

Generation of Alginate Microspheres for Biomedical Applications

Published on: August 12, 2012

22.1K

Efficient functionalization of alginate biomaterials.

Marianne Ø Dalheim1, Julie Vanacker2, Maryam A Najmi1

  • 1NOBIPOL, Department of Biotechnology, Norwegian University of Science and Technology (NTNU), Trondheim N-7491, Norway.

Biomaterials
|December 29, 2015
PubMed
Summary

Researchers developed a new, byproduct-free method for chemically modifying alginates using periodate oxidation and reductive amination. This technique efficiently couples bioactive peptides to alginate scaffolds for regenerative medicine applications.

Keywords:
AlginateCell adhesionPeriodate oxidationRGD peptideReductive aminationTissue engineering

More Related Videos

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.3K
Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
11:16

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Published on: September 9, 2022

3.6K

Related Experiment Videos

Last Updated: Mar 28, 2026

Generation of Alginate Microspheres for Biomedical Applications
10:33

Generation of Alginate Microspheres for Biomedical Applications

Published on: August 12, 2012

22.1K
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

18.3K
Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells
11:16

Fabrication and Use of Dry Macroporous Alginate Scaffolds for Viral Transduction of T Cells

Published on: September 9, 2022

3.6K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Chemical Engineering

Background:

  • Alginates are widely used as scaffold materials in regenerative medicine and tissue engineering.
  • Current chemical functionalization methods, like carbodiimide chemistry, can produce undesirable byproducts.
  • There is a need for alternative, efficient, and reproducible methods for peptide coupling to alginates.

Purpose of the Study:

  • To present an alternative chemical functionalization strategy for alginates.
  • To develop a reproducible protocol for peptide coupling to alginate scaffolds.
  • To evaluate the bioactivity of peptide-coupled alginates in cell adhesion studies.

Main Methods:

  • Partial periodate oxidation of alginate followed by reductive amination.
  • Utilized l-Tyrosine methyl ester as a model compound and pic-BH3 as the reducing agent.
  • Characterized the modified alginates using DOSY and NMR spectroscopy; assessed cell adhesion with C2C12 and RP89 cells.

Main Results:

  • Achieved high and precise degrees of substitution with high reproducibility and no byproducts.
  • Coupling efficiency varied with alginate composition, being highest on mannuronan.
  • Successfully coupled three bioactive peptide sequences (GRGDYP, GRGDSP, KHIFSDDSSE) with degrees of substitution between 3.9% and 6.9%.

Conclusions:

  • The developed periodate oxidation and reductive amination method offers a superior alternative to carbodiimide chemistry for alginate functionalization.
  • Peptide-coupled alginates demonstrate bioactivity, supporting cell adhesion in a peptide concentration-dependent manner.
  • This method provides a versatile platform for creating advanced biomaterials for tissue engineering and regenerative medicine.