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Effect of Ionic and Nonionic Carriers in Electrical Field-Flow Fractionation
Mathuros Ornthai1,2, Atitaya Siripinyanond1, Bruce K Gale3
1Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Mahidol University , Rama VI Rd., Bangkok 10400, Thailand.
Analytical Chemistry
|December 29, 2015
Summary
Electrical Field-Flow Fractionation (ElFFF) can now analyze biological samples by optimizing carrier liquids. Nonionic additives like isopropanol and sucrose enable retention at biologically relevant concentrations, overcoming electrode polarization limitations.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biophysical Chemistry
Background:
- Electrical Field-Flow Fractionation (ElFFF) faces electrode polarization issues with ionic carrier liquids.
- Biological samples often require high ionic strength and osmotic concentrations, posing challenges for ElFFF.
Purpose of the Study:
- To investigate the impact of phosphate buffered saline (PBS), isopropanol (IPA), and sucrose concentrations on ElFFF retention and separation.
- To determine if carrier solution modification can enable ElFFF analysis of biological materials.
Main Methods:
- ElFFF was performed using varying concentrations of PBS, IPA, and sucrose as carrier liquids.
- Elution time, current, conductivity, and channel resistance were monitored.
- DC ElFFF and cyclical ElFFF (CyE1FFF) were employed.
Main Results:
- Nonionic additives IPA and sucrose demonstrated good retention even at high concentrations.
- IPA up to 60% and sucrose up to 0.3 M were effective.
- Biologically isotonic conditions (2% IPA or 0.30 M sucrose) yielded good retention with both DC ElFFF and CyE1FFF.
Conclusions:
- Modifying carrier solutions with PBS, IPA, and/or sucrose can overcome ElFFF limitations for biological samples.
- The use of nonionic additives at biologically relevant concentrations makes ElFFF a viable technique for bioparticle characterization and separation.
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