Platelet lysate-based pro-angiogenic nanocoatings

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Inducing vascularization in 3D constructs is critical for tissue viability and overcoming limitations in construct size.
• Platelet lysate (PL) is a rich, cost-effective source of pro-angiogenic factors like VEGF A and FGF b.
• Previous nanocoatings incorporating PL activated endothelial cells, promoting tube-like structure formation.

Purpose of the Study:

• To explore the use of layer-by-layer assembly with PL and sulfated polysaccharides for inducing neo-vascularization.
• To evaluate the potential of these interfaces to promote cell-based tubular structure formation in 2D/3D constructs.

Main Methods:

• Layer-by-layer assembly was used to create nanocoatings incorporating human platelet lysate (PL) with marine-origin polysaccharides.
• Human umbilical vein endothelial cells (HUVECs) were incubated with the nanocoatings.
• Angiogenic gene expression and tube-like structure formation were analyzed.

Main Results:

• More sulfated nanocoatings induced HUVECs to form tube-like structures within 20 hours.
• Increased expression of angiogenic genes, including angiopoietin-1 and VEGF A, was observed.
• The combination of PL and sulfated polysaccharides demonstrated synergistic pro-angiogenic effects.

Conclusions:

• Combining PL with sulfated polyelectrolytes offers a versatile and cost-effective method to create interfaces for tissue engineering constructs.
• These interfaces can instruct angiogenic cells, promoting the formation of neo-vascularization and cell-based tubular structures.
• This approach holds potential for overcoming challenges in creating large, viable tissue constructs.