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Published on: August 15, 2019
[Identification of Bx02 Alleles and Genetic Analysis of the Involved Ancestry]
Guo-Liang Liu1, Fang Zhu1, Xiao-Li Zheng1
1Henan Provincial Key Engineering Laboratory of Antibody Drugs, School of Medicine, Henan University, Kaifeng 475004, Henan Province, China.
Objective:
To identify the genotypes of the blood sample whose blood grouping showed discrepancies and study the ABO alleles' molecular characteristics of the involved ancestry.
Methods:
Blood samples were preliminary genotyped by PCR-SSP. Complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and cloning sequenced to identify its genotype.
Results:
Sequence analysis indicated that 3 samples of the family had an nt905A>G mutation in the B gene compared with ABO*B101. Combined with the serological results, the propositus could be typed as Bx02/O102.
Conclusion:
DNA sequencing analysis is able to identify the serological phenotype samples that forward and reverse group methods were incongruous.
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