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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
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Biomass accessibility analysis using electron tomography.

Jacob D Hinkle1, Peter N Ciesielski2, Kenny Gruchalla1

  • 1Computational Science Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401 USA.

Biotechnology for Biofuels
|December 29, 2015
PubMed
Summary
This summary is machine-generated.

New methods using 3D electron tomography quantify biomass surface accessibility after pretreatment. Accessibility significantly decreases for larger probe sizes, impacting catalyst access.

Keywords:
AccessibilityBiomassCellulosePorosimetryPretreatmentTomography

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Area of Science:

  • Biomass deconstruction
  • Plant cell wall microstructure
  • Catalyst accessibility

Background:

  • Substrate accessibility is key to biomass deconstruction theories.
  • Current methods lack mechanistic insight into pretreatment-induced accessibility changes.

Purpose of the Study:

  • To develop and apply methods for characterizing surface accessibility based on fine-scale microstructure.
  • To analyze how different pretreatments affect corn stover cell wall accessibility.

Main Methods:

  • Utilized 3D electron tomography to reveal plant cell wall microstructure.
  • Developed a flexible, image-based model for analyzing cell wall architecture and accessibility.
  • Examined native and pretreated (dilute acid, steam explosion, AFEX) corn stover.

Main Results:

  • Introduced a framework for quantifying surface accessibility using image-based microstructural data.
  • Demonstrated that pretreatment significantly alters cell wall architecture and accessibility.
  • Observed a strong dependence of accessibility on probe size.

Conclusions:

  • Image-based measures effectively quantify pretreatment-induced increases in biomass surface accessibility.
  • Biomass surface accessibility dramatically decreases for probe sizes above 5-10 nm radius.
  • Understanding microstructural changes is crucial for optimizing biomass deconstruction.