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Updated: Aug 9, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Purification of labelled antibodies by hydrophobic interaction chromatography
1Department I of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.
Abstract:
In order to improve the sensitivity of immunometric assays, a chromatographic technique was developed that virtually climinates components causing non-specific background. Labelled antibodies were applied to a phenyl-Sepharose column in physiological buffer. When labelled antibodies were purified by this technique, the non-specific background of various time-resolved immunofluorometric assays was reduced 3- to 10-fold and was very close to the instrument background. The assay sensitivity was simultaneously increased by a factor of 2 to 16. This purification method might be used to improve the results of immunometric assays in general.
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