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Published on: April 18, 2019
Generation of Skin Tissue Using Adipose Tissue-Derived Stem Cells
Mustafa Aykut Ozpur1, Ethem Guneren, Halil Ibrahim Canter
1Adiyaman, Istanbul, Kirklareli, and Samsun, Turkey From the Department of Plastic, Reconstructive and Aesthetic Surgery, Government Hospital; the Department of Plastic, Reconstructive and Aesthetic Surgery, Bezmialem Vakif University; the Department of Plastic, Reconstructive and Aesthetic Surgery, Acibadem University; the Department of Haematolgoy, Acibadem University; the Department of Plastic, Reconstructive and Aesthetic Surgery, Government Hospital; and the Department of Histology, Ondokuz Mayis University.
Adipose tissue-derived mesenchymal stem cells combined with keratinocytes on a fibrin scaffold significantly promote skin regeneration. This approach enhances wound healing, leading to substantial skin replacement in defects.
Area of Science:
- Regenerative Medicine
- Tissue Engineering
- Dermatology
Background:
- Mesenchymal stem cells (MSCs) derived from adipose tissue hold potential for tissue regeneration.
- Developing functional skin substitutes is crucial for treating severe skin defects.
Purpose of the Study:
- To evaluate the efficacy of adipose tissue-derived MSCs and keratinocytes on a fibrin scaffold for skin tissue generation.
- To assess the impact of these components on wound healing and skin replacement.
Main Methods:
- A full-thickness skin defect model was created in Wistar albino rats.
- Fibrin scaffolds were prepared with varying combinations of keratinocytes and adipose tissue-derived MSCs.
- Wound healing was assessed macroscopically and microscopically over time.
Main Results:
- Combined application of MSCs and keratinocytes on fibrin scaffolds (Group 5) resulted in near-complete epithelialization with minimal contraction.
- Microscopic analysis revealed increased collagen and vascular volume ratios in groups treated with MSCs.
- Fluorescent microscopy confirmed stem cell differentiation, extracellular matrix synthesis, and neoangiogenesis in Group 5.
Conclusions:
- An autologous fibrin scaffold seeded with adipose tissue-derived MSCs and coated with keratinocytes significantly promotes skin replacement.
- This combination therapy offers a promising strategy for generating functional skin tissue.
