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Updated: Mar 28, 2026
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In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Published on: December 29, 2021
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[Structure and function of histone chaperone FACT]
M T Bondarenko1,2, N V Maluchenko3, M E Valieva3
1Fox Chase Cancer Center, Philadelphia, PA, 19111-2497 United States.
Molekuliarnaia Biologiia
|December 30, 2015
Summary
The FACT protein complex acts as a histone chaperone, crucial for maintaining and remodeling chromatin structure during DNA processes like transcription and replication. It ensures nucleosome integrity and facilitates cell transformation and tumor cell viability.
Area of Science:
- Molecular Biology
- Epigenetics
- Chromatin Dynamics
Context:
- The FACT (Facilitates Chromatin Transcription) complex is a heterodimer protein essential for DNA-dependent cellular processes.
- It functions as a histone chaperone, playing a critical role in chromatin maintenance and modification.
Purpose:
- To elucidate the role of the FACT complex in nucleosome dynamics during DNA replication, transcription, and repair.
- To understand how FACT facilitates nucleosome preservation and remodeling.
Summary:
- FACT interacts with histone H2A-H2B dimers to facilitate DNA uncoiling from nucleosomes during transcription elongation.
- It reduces nucleosome barrier height, aiding RNA polymerase II passage and ensuring nucleosome survival.
- FACT also assists in the recovery of canonical nucleosome structure post-transcription.
Impact:
- FACT is vital for maintaining chromatin structure during essential cellular processes.
- The complex plays a significant role in human cell transformation.
- It is implicated in sustaining the viability of tumor cells, suggesting potential therapeutic relevance.
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