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[Application of gene microarray to screen differential expression genes of Modic changes at vertebral endplates]
Minou Xu1, Zhe Chen, Zezhu Zhou
1Department of Orthopaedics, Zheng Linhai Second People's Hospital, Zhejiang 317016, China.
Objective:
To identify differentially expressed genes in Modic changes by gene microarray method.
Methods:
From August 2014 to December 2014, gene expression profiling analysis of 5 lumbar endplates with Modic changes and 5 control specimens without Modic changes were performed in Department of Orthopaedics, Zheng Linhai Second People's Hospital and Department of Orthopaedics, Shanghai Jiaotong University School of Medicine. The functional analysis (Gene Ontology and KEGG) of deregulated genes was carried out.The qRT-PCR analysis was performed to validate differential expression genes.
Results:
Of 263 significantly differential expression genes (P<0.05, Fold Change > 2), 107 were over-expressed and 156 under-expressed genes.Those deregulated genes were mainly involved in chemotaxis and cell motion. The qRT-PCR analysis of 2 up-regulated genes (CXCL14, KCNMA1) and 4 under-regulated genes (MARCKS, ZEB2, PSMF1, and CNN2) mRNA expression levels validated the results from the microarray analysis.
Conclusions:
There are differentially expressed genes between lumbar endplate with Modic changes and without Modic changes.Modic changes may be multiple genes involved and regulated pathological changes.
