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Human-Mouse Chimerism Validates Human Stem Cell Pluripotency.

Victoria L Mascetti1, Roger A Pedersen1

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Cell Stem Cell
|December 30, 2015
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cells (hPSCs) can contribute to normal mouse development. This study validates the pluripotency of hPSCs, including human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs), through chimera formation.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Pluripotent stem cells (PSCs) possess the potential to differentiate into all cell types.
  • Chimera formation is a critical test for assessing PSC pluripotency.
  • The capacity of human pluripotent stem cells (hPSCs) to form embryonic chimeras has been uncertain.

Purpose of the Study:

  • To functionally validate the pluripotency of hPSCs in vivo.
  • To investigate the developmental potential of hPSCs in interspecies chimera models.
  • To determine if hPSCs can contribute to normal embryonic development.

Main Methods:

  • Transplantation of hPSCs (including hiPSCs and hESCs) into gastrula-stage mouse embryos.
  • Stage-matching of donor and host embryos for optimal integration.
  • Analysis of chimera formation and contribution to embryonic tissues.

Main Results:

  • hPSCs efficiently formed interspecies chimeras with mouse embryos.
  • Transplanted hPSCs contributed to all three primary germ layers.
  • The colonization patterns of hPSCs aligned with classical developmental fate mapping predictions.

Conclusions:

  • hPSCs, including hiPSCs and hESCs, demonstrate functional pluripotency in vivo.
  • Successful interspecies chimera formation validates the developmental potential of hPSCs.
  • Evidence suggests human-mouse interspecies developmental competency is achievable.