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Crystallization of alcohol oxidase from Pichia pastoris
C W Boys1, D J Hill, P G Stockley
1Astbury Department of Biophysics, University of Leeds, U.K.
Journal of Molecular Biology
|July 5, 1989
Abstract:
Crystals of alcohol oxidase purified from Pichia pastoris were grown in microdialysis buttons in a solution of polyethylene glycol, sodium chloride and sodium azide. The crystals were stratified along the major axis and up to 3 mm in length. X-ray diffraction experiments indicated a space group of P2(1) and unit cell dimensions of a = 157.3 A, b = 171.5 A and c = 231.6 A. Crystals diffract to beyond 2.7 A and are suitable for X-ray structure analysis.