Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core
João I Mamede1, Thomas J Hope2
1Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E. Superior, Lurie 9-280, Chicago, IL, 60611, USA.
Methods in Molecular Biology (Clifton, N.J.)
|December 31, 2015
Abstract:
Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.
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