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Updated: Mar 28, 2026
Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Detection of integrons and Staphylococcal Cassette Chromosome (SCCmec) types in Staphylococcus aureus isolated from
Amirmorteza Ebrahimzadeh Namvar1, Farzaneh Khodaei2, Aslan Bijari3
1Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences Babol, Iran.
Background:
Methicillin Resistant Staphylococcus aureus (MRSA) strains have been recognized as an important reason of infections in health care units. Integrons role in antibiotic resistance box gene transfer has been well recognized which are found in Gram positive bacteria.
Objective:
The aim of this study was analyzed of SCCmec typing and determine of integron classes in burn and non-burn specimens.
Methodology:
A total of 110 S. aureus strains were isolated from burn and non-burn patients. Antimicrobial susceptibility testing, detection of mecA gene, various SCCmec types and integrons classes were analyzed.
Results:
In antimicrobial susceptibility test in burn patients, resistant to both gentamicin and oxacilin and in non-burn patients resistance to oxacilin and cefepime showed the highest ratio In PCR molecular test (80%) and (52.7%) of strains harbored the mecA gene. Therefore five different SCCmec types were recognized among our studied strains. Subsequently, integron class I was evaluated as (94.5%) in burn and (12.7%) in non-burn isolates by the multiplex PCR method.
Conclusion:
Albeit MRSA strains have the hospital reservoir so may cause serious treats for hospitalized and non-hospitalized patients, hence clinical decision for prevention and treatment may develop due to, mecA gene, SCCmec elements and integrons detection in health care units.
Insights
Methicillin-resistant Staphylococcus aureus (MRSA) is a significant healthcare threat. This study identified SCCmec types and integron classes in burn and non-burn patients, aiding in infection control strategies.
Area of Science:
- Microbiology
- Infectious Diseases
- Molecular Biology
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of healthcare-associated infections.
- Integrons play a crucial role in the transfer of antibiotic resistance genes in Gram-positive bacteria.
Purpose of the Study:
- To analyze SCCmec typing in Staphylococcus aureus strains.
- To determine the classes of integrons present in burn and non-burn patient isolates.
Main Methods:
- Isolated 110 Staphylococcus aureus strains from burn and non-burn patients.
- Performed antimicrobial susceptibility testing, mecA gene detection, SCCmec typing, and integron class analysis using multiplex PCR.
Main Results:
- High prevalence of mecA gene detected in 80% of burn and 52.7% of non-burn isolates.
- Five distinct SCCmec types were identified.
- Integron class I was highly prevalent in burn isolates (94.5%) compared to non-burn isolates (12.7%).
Conclusions:
- MRSA poses serious risks to both hospitalized and non-hospitalized patients.
- Detection of mecA gene, SCCmec elements, and integrons is crucial for clinical decision-making in healthcare settings for MRSA prevention and treatment.
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