Related Experiment Video
Updated: Mar 28, 2026
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
Published on: July 28, 2016
Relationship between glycemic control and histochemical myeloperoxidase activity in neutrophils in patients with type
Mustafa Unubol1, Irfan Yavasoglu2, Firuzan Kacar3
1Department of Endocrinology, Faculty of Medicine, Adnan Menderes University, 09100 Aydın, Turkey.
Background:
Myeloperoxidase (MPO) is a lysosomal hemoprotein found in the azurophilic granules in neutrophils. Myeloperoxidase plays an important role in oxygen-dependent killing of bacteria, fungi, virus and malignant cells. Diabetes mellitus (DM) is listed among conditions that may lead to secondary MPO deficiency in neutrophils but inconsistent results concerning MPO activity in diabetic patients have been reported in the literature. In this study, we aimed to evaluate the relationship between glycemic control in patients with type 2 DM and MPO activity in neutrophils from a histochemical perspective.
Methods:
The study included 40 patients with type 2 DM with poor glycemic control, 30 patients with type 2 DM with good glycemic control and 31 healthy controls. Peripheral blood smears were analyzed for each patient included in the study. Myeloperoxidase dye was used for staining. Myeloperoxidase ratios in neutrophil were evaluated for proportions of staining with MPO in 100 neutrophils in each smear. SPSS 16.0 version was used for statistical analyses.
Results:
Myeloperoxidase ratios in neutrophils were 70 (58.5-80) in type 2 DM patients with poor glycemic control compared to 80 (73.75-90) in those with good glycemic control and 88 (78-92) in healthy controls. The DM group with poor glycemic control was statistically significantly different from the other groups (p < 0.001).
Conclusions:
Poor glycemic control in diabetic patients results in decreased MPO activity in neutrophils histochemically.
Insights
Poor glycemic control in type 2 diabetes mellitus (DM) is linked to reduced neutrophil myeloperoxidase (MPO) activity. This study investigated the histochemical MPO levels in neutrophils of diabetic patients with varying glycemic control.
Area of Science:
- Immunology
- Endocrinology
- Hematology
Background:
- Myeloperoxidase (MPO) is crucial for neutrophil antimicrobial functions.
- Diabetes mellitus (DM) is associated with potential secondary MPO deficiency, but findings are inconsistent.
- This study examines MPO activity in neutrophils concerning glycemic control in type 2 DM.
Purpose of the Study:
- To evaluate the relationship between glycemic control in type 2 DM patients and neutrophil MPO activity.
- To investigate MPO activity from a histochemical perspective in diabetic neutrophils.
Main Methods:
- Histochemical analysis of peripheral blood smears from 40 type 2 DM patients with poor glycemic control, 30 with good glycemic control, and 31 healthy controls.
- Quantification of MPO ratios in neutrophils using MPO staining.
- Statistical analysis using SPSS 16.0.
Main Results:
- Neutrophil MPO ratios were significantly lower in type 2 DM patients with poor glycemic control (70) compared to those with good glycemic control (80) and healthy controls (88).
- The difference in MPO ratios between the poorly controlled DM group and the other groups was statistically significant (p < 0.001).
Conclusions:
- Poor glycemic control in type 2 diabetes mellitus leads to a histochemically demonstrable decrease in neutrophil MPO activity.
- This finding suggests a potential link between hyperglycemia and impaired neutrophil function in diabetic patients.
