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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Mar 28, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

ChIP-Seq: Library Preparation and Sequencing.

Karyn L Sheaffer¹, Jonathan Schug²

¹Department of Genetics, and Institute for Diabetes, Obesity and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Methods in Molecular Biology (Clifton, N.J.)

|January 2, 2016

Summary

This summary is machine-generated.

Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-Seq) identifies genome-wide DNA-binding patterns. This method isolates chromatin, immunoprecipitates DNA, and uses next-generation sequencing for detailed analysis.

Keywords:

Chromatin immunoprecipitation DNA binding Next-generation sequencing

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Last Updated: Mar 28, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Comparative Lesions Analysis Through a Targeted Sequencing Approach

Published on: November 5, 2019

3' End Sequencing Library Preparation with A-seq2

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Published on: October 10, 2017

Area of Science:

Molecular Biology
Genomics
Epigenetics

Background:

Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-Seq) is a key technique in molecular biology.
It is widely used to map the locations of DNA-binding factors across the genome.
Applications include studying transcription factors, histone modifications, and transcriptional activity.

Purpose of the Study:

To describe detailed methods for performing ChIP-Seq.
To outline the process of isolating chromatin from tissues.
To explain the immunoprecipitation of DNA bound to specific proteins.

Main Methods:

Isolation of chromatin from biological tissues.
Immunoprecipitation of DNA associated with a protein of interest.
Next-generation sequencing for genome-wide DNA-binding pattern identification.

Main Results:

Successful identification of genome-wide DNA-binding patterns.
Provides a comprehensive approach to analyzing protein-DNA interactions.
Enables assessment of regulatory input and epigenetic modifications.

Conclusions:

The described ChIP-Seq methods are effective for determining genome-wide DNA-binding patterns.
This technique is crucial for understanding gene regulation and epigenetic landscapes.
The protocol facilitates the study of various DNA-binding factors and their roles.