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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Analyzing MiRNA-LncRNA Interactions.

Maria D Paraskevopoulou1, Artemis G Hatzigeorgiou2

  • 1DIANA-Lab, Department of Electrical & Computer Engineering, University of Thessaly, Volos, Greece. mparaskevopoulou@uth.gr.

Methods in Molecular Biology (Clifton, N.J.)
|January 2, 2016
PubMed
Summary
This summary is machine-generated.

Long noncoding RNAs (lncRNAs) act as miRNA sponges, sequestering microRNAs (miRNAs) and influencing gene regulation. This study explores computational and experimental methods for analyzing these complex miRNA-lncRNA interactions.

Keywords:
Experimentally supportedHITS-CLIPIn-silico predictionsInteractionPAR-CLIPSpongelncRNAmicroRNA

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Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Long noncoding RNAs (lncRNAs) are a large, diverse class of RNA molecules with largely uncharacterized functions.
  • MicroRNAs (miRNAs) are key regulators of gene expression at the posttranscriptional level.
  • lncRNAs can function as miRNA sponges, modulating miRNA activity and impacting gene regulatory networks.

Purpose of the Study:

  • To investigate the interactions between long noncoding RNAs and microRNAs.
  • To present methodologies for analyzing miRNA-lncRNA interactions using in silico and experimental approaches.
  • To provide a foundation for advanced functional miRNA analyses.

Main Methods:

  • In silico analysis of potential miRNA-lncRNA interactions.
  • Experimental validation of predicted miRNA-lncRNA interactions.
  • Development of multistep functional miRNA analysis frameworks.

Main Results:

  • Identification of lncRNAs acting as miRNA sponges.
  • Characterization of the regulatory impact of lncRNA-miRNA interactions on gene expression.
  • Validation of computational predictions through experimental data.

Conclusions:

  • lncRNA-miRNA interactions represent a significant layer of gene regulation.
  • The presented methodologies facilitate the study of these complex interactions.
  • These approaches can be adapted for comprehensive functional miRNA analysis.