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Published on: March 30, 2019
Effect of miR-340 on gastric cancer cell proliferation and apoptosis
1Department of General Surgery, The First Affiliated Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
Abstract:
Gastric cancer pathogenesis is a multi-factor, multi-step, complicated process that related to gene abnormal expression. This study intended to explore the miR-340 effect on human gastric cancer cell line SGC-7901 and BGG823 proliferation and apoptosis, as to provide theoretical basis and experimental evidence for potential clinical application. Array was used to screen gastric cancer related abnormal genes. Q-PCR was applied to detect the screened genes expression in tissue and gastric cancer cells. MTT and colony formation assay were performed to evaluate miR-340 impact on gastric cancer proliferation. Flow cytometry was used to determine cell cycle and cell apoptosis. Q-PCR showed that miR-340 overexpressed in gastric cancer tissue significantly compared with normal control (P < 0.01). MiR-340 overexpression can promote SGC-7901 and BGC823 cells proliferation with 50% proliferation rate. Soft agar colony formation assay also showed that miR-340 overexpression can facilitate gastric cancer cell proliferation. Cell cycle analysis revealed that miR-340 overexpression can reduce cell apoptosis. Annexin V/PI staining demonstrated that miR-340 transfection can decrease cell apoptotic rate (4.58%, 1.98%, 2.11%). MiR-340 can promote tumor cell growth and reduce cell apoptosis effectively.
Insights
MicroRNA-340 (miR-340) is overexpressed in gastric cancer, promoting tumor cell growth and reducing apoptosis. This finding offers insights into gastric cancer progression and potential therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gastric cancer pathogenesis involves complex genetic alterations and abnormal gene expression.
- MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
- Understanding the specific roles of miRNAs like miR-340 is vital for potential clinical applications.
Purpose of the Study:
- To investigate the effect of miR-340 on the proliferation and apoptosis of human gastric cancer cell lines (SGC-7901 and BGG823).
- To provide theoretical and experimental evidence for the potential clinical application of targeting miR-340 in gastric cancer.
Main Methods:
- Gene expression screening using array technology.
- Quantitative Polymerase Chain Reaction (Q-PCR) for gene expression analysis in tissues and cells.
- MTT and colony formation assays to assess cell proliferation.
- Flow cytometry and Annexin V/PI staining for cell cycle and apoptosis analysis.
Main Results:
- Q-PCR confirmed significant overexpression of miR-340 in gastric cancer tissues compared to normal controls (P < 0.01).
- Overexpression of miR-340 promoted proliferation in SGC-7901 and BGG823 cells, evidenced by MTT and colony formation assays.
- Cell cycle analysis and Annexin V/PI staining indicated that miR-340 overexpression reduced cell apoptosis, with decreased apoptotic rates observed.
Conclusions:
- MiR-340 overexpression effectively promotes gastric cancer cell growth.
- MiR-340 plays a role in inhibiting apoptosis in gastric cancer cells.
- These findings highlight miR-340 as a potential therapeutic target for gastric cancer treatment.
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