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tRNA conjugation with chitosan nanoparticles: An AFM imaging study.
D Agudelo1, L Kreplak2, H A Tajmir-Riahi1
1Department of Chemistry-Biochemistry and Physics, University of Québec at Trois-Rivières, C. P. 500, Trois-Rivières (Québec), G9A 5H7, Canada.
Chitosan nanoparticles bind to transfer RNA (tRNA), forming stable conjugates. Larger chitosan sizes result in stronger binding, with tRNA structure largely preserved despite chitosan-induced changes.
Area of Science:
- Biochemistry
- Materials Science
- Nanotechnology
Background:
- Transfer RNA (tRNA) is crucial for protein synthesis.
- Chitosan nanoparticles are biocompatible materials with potential biomedical applications.
Purpose of the Study:
- To investigate the conjugation of tRNA with chitosan nanoparticles of varying sizes.
- To elucidate the binding interactions and structural consequences of this conjugation.
Main Methods:
- Spectroscopic methods (UV-Vis, fluorescence) were employed.
- Atomic Force Microscopy (AFM) was used for structural analysis.
- Binding constants were determined for different chitosan sizes.
Main Results:
- Chitosan binds tRNA through electrostatic, hydrophilic, and hydrogen bonding interactions.
- Binding affinity increases with chitosan nanoparticle size (15, 100, 200 kDa).
- Chitosan induces tRNA aggregation and particle formation, while preserving the A-family structure.
Conclusions:
- Chitosan nanoparticles can effectively conjugate with tRNA.
- The size of chitosan nanoparticles influences the stability and binding affinity of the conjugate.
- This interaction has implications for developing novel nucleic acid delivery systems.
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