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Related Concept Videos

Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
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Methods of Sterilization II: Chemical Methods01:30

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
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Physical Methods for Controlling Microbial Growth: Temperature01:23

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Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
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Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
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Thermal strain is a concept that arises when we consider how temperature changes affect structures. Unlike the conventional assumption that structures remain constant under load, real-world scenarios often involve temperature fluctuations that can significantly impact these structures. Consider a homogeneous rod with a uniform cross-section resting freely on a flat horizontal surface. If the rod's temperature increases, the rod elongates. This elongation is proportional to the temperature...
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Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
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Physical structure changes of solid medium by steam explosion sterilization.

Zhi-Min Zhao1, Lan Wang2, Hong-Zhang Chen2

  • 1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.

Bioresource Technology
|January 3, 2016
PubMed
Summary
This summary is machine-generated.

Steam explosion sterilization physically alters solid media structure, increasing pore size and surface area. This enhances microbial contact, significantly improving solid-state fermentation (SSF) performance through both physical and chemical effects.

Keywords:
Low-field nuclear magnetic resonance (LF-NMR)MicrostructureSolid mediumSolid-state fermentationSteam explosion sterilization

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Area of Science:

  • Biotechnology
  • Materials Science
  • Microbiology

Background:

  • Solid-state fermentation (SSF) is crucial for various bioprocesses.
  • Optimizing the physical and chemical properties of solid media is key to enhancing SSF efficiency.
  • Steam explosion sterilization is a potential method for modifying solid media.

Purpose of the Study:

  • To investigate the physical structural changes in solid media induced by steam explosion sterilization.
  • To elucidate the mechanisms by which these structural changes impact solid-state fermentation (SSF) performance.
  • To determine the combined physical and chemical effects of steam explosion sterilization on SSF.

Main Methods:

  • Analysis of molecular and three-dimensional structural changes in solid media post-steam explosion.
  • Measurement of capillary water relaxation time and specific surface area.
  • Correlation of structural parameters with SSF performance metrics.

Main Results:

  • Steam explosion significantly altered the solid medium's molecular and 3D structure.
  • Hydrophilic groups were exposed, and pores/cavities were enlarged.
  • Capillary water-filled pores were identified as active sites for SSF, linked to relaxation time, surface area, and fermentation outcomes.
  • Effective contact area for microbial cells increased, boosting SSF performance.

Conclusions:

  • Steam explosion sterilization enhances SSF by modifying the physical structure of solid media.
  • The improvement in SSF is attributed to increased effective surface area and optimized pore structure.
  • Mechanisms of SSF enhancement involve both physical alterations and potential chemical changes induced by steam explosion.