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Properties of retroviral protease responsible for gag precursor cleavage
R Hafenrichter1, F Weiland, J Schneider
1Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Virology
|September 1, 1989
Abstract:
Friend Leukemia Virus (FLV) particles contain a protease which cleaves not only its own gag precursor but also the gag polyprotein of simian sarcoma virus (SSV). To determine the localization of the enzyme within the virion, purified virus was fractionated. According to our studies most of the proteolytic activity is located within the retroviral core. Since ionic detergents inhibit the protease, heat treatment was found to be the most effective way to release the molecule from the virus particle. The in vitro studies indicate a high resistance of the enzyme to different kinds of heat treatment.