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Early and late effects of Ibuprofen on mouse sperm parameters, chromatin condensation, and DNA integrity in mice
Fatemeh Roodbari1, Nahid Abedi1, Ali Reza Talebi2
1Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Sari, Iran.
Background:
There are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and DNA integrity.
Objective:
To determine the effects of Ibuprofen on sperm parameters, chromatin condensation and DNA integrity of mice.
Materials And Methods:
In this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control (group I, n=12), normal dosage of ibuprofen (group II, n=12) and high dosage (group III, n=12). Ibuprofen with different doses was dissolved in daily water of animals. After 35, 70 and 105 days, the cauda epididymis of mice were cut and incubated in Ham's F10 media. Sperm samples were analyzed for parameters (motility, morphology and count), DNA integrity (SCD test) and chromatin condensation (chromomycin A3 and Aniline blue staining).
Results:
After 35 days, in addition to above mentioned sperm parameters, all of the treated mice showed statistically significant increase in spermatozoa with immature chromatin (P<0.05). However, after 70 days, the rate of sperm DNA fragmentation assessed by SCD was increased in group II (66.5±0.7) and the percentage of immature spermatozoa (AB(+) and CMA3(+)) was higher in group III (77.5±0.7 and 49.5±6.3 respectively) than other groups. After 105 days, the AB(+) spermatozoa were increased in both normal dose and high dose groups.
Conclusion:
Ibuprofen may cause a significant reduction in sperm parameters and sperm chromatin/DNA integrity in mice. It should be noted that these deleterious effects are dose-dependent and can be seen in early and late stage of drug treatments.
Insights
Ibuprofen negatively impacts mouse sperm quality, motility, and DNA integrity. These adverse effects are dose-dependent and observed with both short-term and long-term exposure.
Area of Science:
- Reproductive Toxicology
- Pharmacology
- Sperm Biology
Background:
- Limited research exists on ibuprofen's adverse effects on sperm fertility and DNA.
- Investigating non-steroidal anti-inflammatory drugs' impact on reproductive health is crucial.
Purpose of the Study:
- To evaluate the effects of ibuprofen on sperm parameters, chromatin condensation, and DNA integrity in mice.
- To assess dose-dependent and time-dependent impacts of ibuprofen exposure.
Main Methods:
- Adult male mice were divided into control, normal-dose ibuprofen, and high-dose ibuprofen groups.
- Sperm samples were analyzed for motility, morphology, count, DNA integrity (SCD test), and chromatin condensation (Aniline blue and chromomycin A3 staining) after 35, 70, and 105 days.
Main Results:
- Ibuprofen exposure led to increased immature spermatozoa after 35 days.
- After 70 days, significant sperm DNA fragmentation and immature chromatin were observed in ibuprofen-treated groups.
- Elevated levels of immature spermatozoa persisted up to 105 days in both normal and high-dose groups.
Conclusions:
- Ibuprofen significantly reduces sperm parameters and compromises sperm chromatin/DNA integrity in mice.
- The detrimental effects of ibuprofen on sperm are dose-dependent.
- Adverse impacts on sperm are evident in both early and late stages of ibuprofen treatment.
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