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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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Pseudomonas aeruginosa serotypes and resistance to antibiotics from wound swabs

Natasa Stanković Nedeljković, Branislav Tiodorović, Branislava Kocić

    Vojnosanitetski Pregled
    |January 7, 2016
    PubMed
    Abstract

    Insights

    Pseudomonas aeruginosa is a common cause of wound infections. This study found high sensitivity to colistin and meropenem, with low resistance in wound isolates, indicating effective treatment options.

    Area of Science:

    • Microbiology
    • Infectious Diseases
    • Clinical Research

    Background:

    • Pseudomonas aeruginosa (P. aeruginosa) is a leading cause of wound infections, necessitating an understanding of its antibiotic susceptibility.
    • Assessing antibiotic resistance patterns is crucial for effective wound infection management.

    Purpose of the Study:

    • To analyze the prevalence of P. aeruginosa in wound swabs.
    • To determine antibiotic susceptibility, minimum inhibitory concentrations (MICs), and metallo-β-lactamases (MBLs) production.
    • To identify common P. aeruginosa serotypes and their associated resistance profiles.

    Main Methods:

    • Cultivation of wound swabs from 90 outpatients and 55 inpatients.
    • Antibiotic susceptibility testing against meropenem, imipenem, piperacillin-tazobactam, ceftazidime, cefepime, amikacin, gentamicin, netilmicin, ofloxacin, ciprofloxacin, and colistin.
    • Serotyping using polyvalent and monovalent antisera for agglutination.

    Main Results:

    • P. aeruginosa was isolated from 36.55% of wound swabs.
    • Highest sensitivity observed for colistin (100%) and meropenem (93.44%); lowest sensitivity to cefepime (19.54%).
    • Prevalence of MBLs production was 9.43%; common serotypes included P11 (22.64%), P6 (15.09%), and P1 (11.32%).

    Conclusions:

    • No significant increase in P. aeruginosa presence or antibiotic resistance was noted in nosocomial versus outpatient isolates.
    • Colistin and meropenem demonstrated the highest efficacy against the tested P. aeruginosa isolates.
    • Cefepime showed the lowest efficacy, highlighting the need for targeted antibiotic selection in wound infections.

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