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A single-tube confirmatory test for E. coli using brilliant green mannitol methylumbelliferyl-beta-d-glucuronide
1Zentrum der Hygiene, Klinikum der J. W. Goethe-Universität Frankfurt a.M., Abteilung für Allgemeine und Umwelthygiene.
Zentralblatt Fur Hygiene Und Umweltmedizin = International Journal of Hygiene and Environmental Medicine
|August 1, 1989
Abstract:
The paper briefly describes the development of a third marker in the single tube technique used in the confirmation of E. coli in water. It has been shown that the third marker, is totally compatible with the markers of gas production from mannitol and indole from tryptophane. The principle of the third marker is to utilise the E. coli specific enzyme, beta-d-glucuronidase, which produces the photoluminescent methylumbelliferone.