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Chromatographic methods and selective detectors in pharmacokinetics
Bollettino Chimico Farmaceutico
|February 1, 1989
Summary
Accurate drug quantification in biological samples is crucial for therapeutic monitoring. This review covers detectors and derivatization for chromatographic analysis of drugs at active concentrations.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Biochemistry
Background:
- Quantitative drug evaluation in biological samples is essential for therapeutic drug monitoring.
- Pharmacologically active drug concentrations range from ng/mL to µg/mL in plasma.
- Chromatographic techniques are the standard for drug analysis in biological matrices.
Purpose of the Study:
- To review commonly used detectors in Gas-Liquid Chromatography (GLC) and High-Performance Liquid Chromatography (HPLC).
- To discuss the selective performance of these detectors for drug analysis.
- To highlight derivatization reactions crucial for quantifying drugs at therapeutic concentrations.
Main Methods:
- Review of literature on chromatographic detectors (GLC and HPLC).
- Analysis of detector selectivity and performance characteristics.
- Examination of derivatization strategies for enhancing drug detectability.
Main Results:
- Identification of frequently employed detectors in GLC and HPLC systems.
- Evaluation of the selective capabilities of various detectors for drug analysis.
- Summary of key derivatization reactions used to improve sensitivity and specificity.
Conclusions:
- Detector choice and derivatization are critical for accurate drug quantification in biological samples.
- Understanding detector performance aids in selecting optimal methods for therapeutic drug monitoring.
- Derivatization techniques enhance the ability to measure drugs at low, pharmacologically relevant concentrations.