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A new direct transfer protocol for cryopreserved IVF embryos.

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Directly transferring frozen in vitro-produced (IVP) embryos with sexed semen offers practical dairy farm logistics. Conception rates were comparable to vitrified embryos, making direct transfer a viable option for increasing the efficiency of IVP embryo utilization.

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BovineDirect transferIVP embryoPregnancy

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Area of Science:

  • Reproductive Biology
  • Animal Science
  • Embryology

Background:

  • Growing demand for sexed in vitro-produced (IVP) embryos in dairy farming.
  • Need for efficient direct embryo transfer protocols to improve IVP embryo utilization.

Purpose of the Study:

  • Compare pregnancy rates of fresh, vitrified, or frozen/directly transferred IVP dairy cow embryos.
  • Evaluate the feasibility of direct embryo transfer for sexed IVP embryos.

Main Methods:

  • In vitro maturation (IVM) and in vitro fertilization (IVF) of Girolando oocytes using sexed semen.
  • Culture of embryos to blastocyst stage over 7 days.
  • Random assignment of Grade I blastocysts to fresh transfer, vitrification/thawing, or direct frozen transfer groups.

Main Results:

  • Conception rates: Fresh embryos (51.35%), vitrified embryos (35.89%), and frozen/directly transferred embryos (40.19%).
  • Direct transfer of frozen IVP embryos achieved conception rates similar to vitrified embryos.
  • No significant difference was found between frozen direct transfer and vitrified embryo transfer (P > 0.05).

Conclusions:

  • Direct transfer of frozen IVP embryos with sexed semen is a practical and logistically simpler alternative to vitrification for dairy farms.
  • This method provides comparable conception rates to vitrified embryos, enhancing the efficiency of IVP embryo application.