Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

26.0K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
26.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.7K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.7K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of static quenching in the photoredox activity of perylene diimide radical anions: implications for consecutive photoinduced electron transfer photocatalysis.

Chemical science·2026
Same author

Quantitative evaluation of light-to-heat conversion and singlet oxygen generation efficiencies on ligand protected gold nanoclusters upon near-infrared excitation.

Nanoscale·2026
Same author

Influence of the substitution pattern on exciton localisation in centrosymmetric quadrupolar dyes.

Physical chemistry chemical physics : PCCP·2026
Same author

When Dielectric Constants Deceive: Interrogating Solvation in Ionic Liquids with Cyclic Voltammetry.

The journal of physical chemistry. B·2026
Same author

Boosting Linear and Nonlinear Optical Properties of Pt<sub>1</sub>Ag<sub>18</sub> Nanoclusters by Manipulating Ligand-Shell Rigidity.

Angewandte Chemie (International ed. in English)·2026
Same author

Ovalene Photophysics Revisited.

The journal of physical chemistry. A·2026

Related Experiment Video

Updated: Mar 27, 2026

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
09:43

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

9.9K

Fluorescent DNA probes at liquid/liquid interfaces studied by surface second harmonic generation.

Giuseppe Licari1, Pierre-François Brevet2, Eric Vauthey1

  • 1Department of Physical Chemistry, University of Geneva, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland. eric.vauthey@unige.ch.

Physical Chemistry Chemical Physics : PCCP
|January 8, 2016
PubMed
Summary

Oxazole yellow dyes exhibit distinct aggregation behaviors at interfaces. Dicationic dyes like YOYO-1 and YOPRO-1 show reduced aggregation, while monocationic dyes form H-aggregates, impacting their interfacial probe potential.

More Related Videos

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.3K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

23.0K

Related Experiment Videos

Last Updated: Mar 27, 2026

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
09:43

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

9.9K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

13.3K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

23.0K

Area of Science:

  • Physical Chemistry
  • Interface Science
  • Spectroscopy

Background:

  • Oxazole yellow dyes are fluorescent probes with applications in various fields.
  • Understanding dye aggregation at interfaces is crucial for optimizing probe performance.
  • Surface Second Harmonic Generation (SSHG) is a sensitive technique for studying interfacial phenomena.

Purpose of the Study:

  • To investigate the interfacial properties and aggregation behavior of oxazole yellow dyes.
  • To compare the behavior of dicationic (YOYO-1, YOPRO-1) and monocationic (YOSAC-1, YOSAC-3) dyes at the dodecane/water interface.
  • To elucidate the factors influencing dye aggregation and excited-state dynamics at interfaces.

Main Methods:

  • Stationary and time-resolved Surface Second Harmonic Generation (SSHG) spectroscopy.
  • Quantum chemical calculations.
  • Polarization-resolved SSHG experiments to determine dye orientation.

Main Results:

  • Dicationic dyes (YOYO-1, YOPRO-1) showed minimal H-aggregation at the interface, unlike their behavior in solution.
  • Monocationic YOSAC dyes exhibited significant H-aggregation at the interface, more so than in solution.
  • Dye orientation at the interface differed, with dicationic dyes lying flatter than monocationic ones.
  • Interfacial interactions in YOYO-1 and YOPRO-1 inhibited non-radiative decay, leading to longer excited-state lifetimes.

Conclusions:

  • Interfacial aggregation behavior of oxazole yellow dyes is dependent on their charge and structure.
  • Dye orientation at the interface plays a key role in aggregation and photophysical properties.
  • These findings highlight the potential of YOYO-1 and YOSAC dyes as specialized fluorescent or SHG interfacial probes.