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Legionella spp. isolation and quantification from greywater.

Sara Rodríguez-Martínez1, Marina Blanky2, Eran Friedler2

  • 1Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa, Israel.

Methodsx
|January 8, 2016
PubMed
Summary

This study modifies the ISO 11731:1998 protocol for detecting Legionella bacteria in greywater (GW). Enhanced methods, including pre-filtration and acid-thermic treatment, improve Legionella isolation from this challenging water source.

Keywords:
Acid-thermic treatmentCultureGreywaterISO 11731:1998Legionella isolationPre-filtration

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Area of Science:

  • Environmental microbiology
  • Water quality monitoring
  • Public health

Background:

  • Legionella is an opportunistic pathogen transmitted via contaminated aerosols, commonly found in water sources.
  • Existing ISO protocols for Legionella monitoring are unsuitable for greywater (GW) due to high bacterial loads.
  • Greywater reuse for applications like toilet flushing poses a risk of Legionella infection through aerosolization.

Purpose of the Study:

  • To develop a modified protocol for effective isolation and quantification of Legionella in greywater.
  • To address the challenges posed by high contaminant bacteria loads in greywater samples.

Main Methods:

  • Modification of the ISO 11731:1998 protocol.
  • Inclusion of a pre-filtration step to remove coarse matter from greywater.
  • Application of a combined acid-thermic treatment to reduce high loads of non-Legionella bacteria.

Main Results:

  • The modified protocol enables successful isolation of Legionella from greywater samples.
  • The pre-filtration and acid-thermic treatment effectively reduce interfering bacteria, facilitating Legionella detection.

Conclusions:

  • The described modifications provide a reliable method for Legionella monitoring in greywater.
  • This improved method is crucial for ensuring the safety of greywater reuse and preventing Legionella infections.