Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Conjugation Reactions: Overview01:14

Phase II Conjugation Reactions: Overview

1.2K
Conjugation, a key component of phase II biotransformation reactions, is a vital process in drug detoxification. It involves transferring endogenous substances like glucuronic acid, sulfate, and glycine to drugs or their metabolites formed in phase I reactions. These conjugation reactions, often catalyzed by specific enzymes, transform potentially harmful metabolites into inactive, water-soluble forms easily excreted in urine or bile. By enhancing polarity and eliminating pharmacological...
1.2K
Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

871
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
871
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

88.9K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
88.9K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

6.4K
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sustained efficacy of dupilumab in pediatric patients with moderate-to-severe atopic dermatitis over 1 year.

The British journal of dermatology·2026
Same author

The Food and Microbiome Longitudinal Investigation (FAMiLI) Study: an Asian American (AsA) Enriched Multi-ethnic Environmental Cohort.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026
Same author

Baricitinib for Itch-Dominant Atopic Dermatitis: A 52-week Baricitinib Real-World Experience in Korea.

Annals of dermatology·2026
Same author

Real-World Efficacy and Safety of Upadacitinib in Korean Patients With Atopic Dermatitis.

Allergy, asthma & immunology research·2026
Same author

Rational Selection of Minimal Sensor Arrays for Analyte Fingerprinting.

Analytical chemistry·2026
Same author

Design of MOSAAIC (Multi-Ethnic Observational Study in American Asian and Pacific Islander Communities).

JACC. Asia·2026

Related Experiment Video

Updated: Mar 27, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.6K

Protein-targeted corona phase molecular recognition.

Gili Bisker1, Juyao Dong1, Hoyoung D Park1

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.

Nature Communications
|January 9, 2016
PubMed
Summary

Researchers developed a new method using single-walled carbon nanotubes (SWCNT) to detect fibrinogen, a key blood protein. This technique shows high selectivity and works in serum, offering potential for medical diagnostics.

More Related Videos

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro
05:50

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro

Published on: September 26, 2025

2.0K
Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

724

Related Experiment Videos

Last Updated: Mar 27, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes
09:28

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

8.6K
Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro
05:50

Pharmacophore Modeling for Targets with Extensive Ligand Libraries: A Case Study on SARS-CoV-2 Mpro

Published on: September 26, 2025

2.0K
Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection
10:02

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

724

Area of Science:

  • Nanotechnology
  • Biomolecular Recognition
  • Materials Science

Background:

  • Corona phase molecular recognition (CoPhMoRe) utilizes nanoparticle-templated heteropolymers for analyte detection.
  • Previous CoPhMoRe applications have been limited to smaller molecules, excluding larger biomacromolecules like proteins.

Purpose of the Study:

  • To adapt and apply the CoPhMoRe technique for the selective recognition of human blood proteins.
  • To investigate the potential of single-walled carbon nanotubes (SWCNT) as a platform for protein detection.

Main Methods:

  • Development of a modified CoPhMoRe screening procedure using SWCNT.
  • Testing the SWCNT-based system against a panel of human blood proteins.
  • Analysis of SWCNT fluorescence quenching upon protein binding and kinetics.

Main Results:

  • Identification of a specific corona phase on SWCNT that selectively recognizes fibrinogen.
  • Observed a significant decrease (>80%) in SWCNT fluorescence upon fibrinogen binding.
  • Evidence suggesting sequential binding of fibrinogen sub-domains and validation through binding kinetics.
  • Demonstrated fibrinogen recognition in a serum environment at clinically relevant concentrations.

Conclusions:

  • The developed SWCNT-based CoPhMoRe variant enables highly selective detection of fibrinogen in complex biological samples.
  • This approach offers a promising foundation for creating synthetic, non-biological antibody analogues for macromolecule recognition.
  • The findings hold significant potential for advancing medical diagnostics and clinical applications.