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Extraction and purification methods in downstream processing of plant-based recombinant proteins
Ewelina Łojewska1, Tomasz Kowalczyk1, Szymon Olejniczak1
1Department of Genetics and Plant Molecular Biology and Biotechnology, The University of Lodz, Banacha Street 12/16, 90-237 Lodz, Poland.
Protein Expression and Purification
|January 9, 2016
Summary
Producing plant-based recombinant proteins is challenging due to high costs and purification difficulties. This review summarizes key downstream processing steps and recent advancements in plant expression systems.
Area of Science:
- Biotechnology
- Plant Molecular Biology
- Biopharmaceutical Production
Background:
- Recombinant protein production in plants has gained significant attention over the last 20 years.
- Proteins are crucial biopharmaceuticals, but their production faces challenges.
- High costs and difficulties in scaling up purification and isolation processes hinder plant-based recombinant protein production.
Purpose of the Study:
- To summarize downstream processing in plant systems.
- To provide an overview of key steps like extraction and purification.
- To highlight recent progress and new developments in downstream technology for plant-based protein production.
Main Methods:
- Review of existing literature on downstream processing in plant systems.
- Focus on extraction and purification techniques.
- Inclusion of both popular and alternative methods for protein isolation.
Main Results:
- Downstream processing of plant-based recombinant proteins involves critical steps like extraction and purification.
- Recent advancements in downstream technology are crucial for overcoming production challenges.
- A variety of established and novel techniques are available for efficient protein recovery.
Conclusions:
- Efficient downstream processing is essential for the successful and cost-effective production of plant-based recombinant proteins.
- Continued innovation in purification and isolation technologies is vital for advancing biopharmaceutical manufacturing in plants.
- Understanding and applying diverse downstream methods can optimize the yield and quality of recombinant proteins from plant systems.
Keywords:
Downstream processingExtraction of proteinPlant based platformsPurification of proteinRecombinant proteinMore Related Videos
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