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Inversion of hematocrit partition at microfluidic bifurcations
Zaiyi Shen1, Gwennou Coupier1, Badr Kaoui2
1Laboratoire Interdisciplinaire de Physique (LIPhy) UMR5588 CNRS-Université Grenoble Alpes, Grenoble F-38041, France.
Microvascular Research
|January 9, 2016
Summary
Red blood cell (RBC) partitioning in microcirculation depends on RBC deformability. Unexpected hematocrit levels in child branches can occur, impacting oxygen delivery.
Area of Science:
- Physiology
- Biophysics
- Microfluidics
Background:
- Red blood cell (RBC) partitioning at microcirculatory bifurcations influences physiological functions but is poorly understood.
- The Zweifach-Fung effect describes RBC distribution based on flow rates in daughter branches.
Purpose of the Study:
- To investigate RBC partitioning in T-shaped microfluidic bifurcations.
- To understand the influence of hematocrit and RBC deformability on partitioning.
- To identify deviations from the established Zweifach-Fung effect.
Main Methods:
- Computer simulations of fluid dynamics.
- In vitro experiments using microfluidic devices.
- Analysis of hematocrit and RBC distribution.
Main Results:
- Hematocrit partitioning strongly depends on RBC deformability at physiological concentrations (<20%).
- Complete RBC deprivation in a daughter branch is possible.
- Observed inverse of the Zweifach-Fung effect at low hematocrit, with higher concentration in lower flow branches.
- RBC transport is highly sensitive to geometrical and cell mechanical properties.
Conclusions:
- RBC partitioning is complex and influenced by cell properties and microchannel geometry.
- Deviations from the Zweifach-Fung effect can occur, impacting microcirculatory function.
- Findings have implications for oxygen delivery in both healthy and diseased states.
