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Expression of FGFs during early mouse tongue development
Wen Du1, Jan Prochazka2, Michaela Prochazkova2
1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, 610041, China; Department of Orofacial Sciences and Program in Craniofacial Biology, University of California San Francisco, San Francisco, CA 94143, USA.
Gene Expression Patterns : GEP
|January 10, 2016
Summary
Fibroblast growth factors (FGFs) are crucial for tongue formation. This study mapped FGF expression during mouse tongue development, revealing FGF signaling regulates multiple stages of this complex process.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Fibroblast growth factors (FGFs) represent a large family of signaling proteins.
- Specific FGF ligands and receptors are implicated in embryonic development, including craniofacial morphogenesis.
- Understanding FGF roles in tongue development is essential for comprehending congenital anomalies.
Purpose of the Study:
- To comprehensively map the expression patterns of all 22 FGFs during mouse tongue development.
- To establish a foundational dataset for future research on FGF involvement in tongue formation.
- To identify specific FGFs and their expression domains critical for embryonic tongue development.
Main Methods:
- Quantitative PCR (qPCR) was employed to measure transcript levels of all 22 FGF genes.
- In situ hybridization was utilized to spatially map FGF expression patterns within the developing mouse tongue.
- Analysis was conducted across a critical developmental window, from embryonic day (E) 11.5 to E14.5.
Main Results:
- Multiple FGFs, including Fgf5, Fgf6, Fgf7, Fgf9, Fgf10, Fgf13, Fgf15, Fgf16, and Fgf18, showed distinct mesenchymal expression.
- Fgf1 and Fgf2 exhibited expression in both epithelial and mesenchymal tissues of the developing tongue.
- Varied transcript levels and spatial expression patterns were observed for different FGFs throughout the studied developmental period.
Conclusions:
- FGF signaling plays a significant and multifaceted role in regulating mouse tongue development.
- The identified FGF expression profiles provide critical insights into the molecular mechanisms governing tongue morphogenesis.
- This study lays the groundwork for investigating FGF-related signaling pathways in normal and aberrant tongue development.
