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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
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Related Experiment Video

Updated: Mar 27, 2026

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
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A multifunctional contrast dye for morphological research.

Qian Xia1,2, Yuanbo Feng2, Ting Yin2

  • 1Department of Nuclear Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Microscopy Research and Technique
|January 11, 2016
PubMed
Summary

A novel red iodized oil (RIO) contrast agent enables multi-modal imaging including X-ray, CT, and MRI. This multifunctional dye aids in visualizing organ anatomy and validating animal experiments, proving effective for tissue vascularity assessment.

Keywords:
Lipiodoldigital radiographymagnetic resonance imagingoil red orabbitrat

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Area of Science:

  • Biomedical Imaging
  • Radiology
  • Materials Science

Background:

  • Developing multifunctional contrast agents is crucial for advanced diagnostic imaging.
  • Current agents often lack versatility across different imaging modalities.
  • There is a need for agents that can also serve in ex vivo validation.

Purpose of the Study:

  • To devise and test a multifunctional contrast dye agent for X-ray based digital radiography (DR) or computer tomography (CT), magnetic resonance imaging (MRI), and colored staining.
  • To evaluate the agent's utility in ex vivo validation of animal experiments.

Main Methods:

  • A custom-formulated red iodized oil (RIO) was prepared by solubilizing Oil Red O in iodized poppy seed oil (Lipiodol).
  • Physicochemical characterizations and imaging studies were performed on normal rats and rabbits with myocardial infarction (MI).
  • RIO was infused, and organs were imaged using clinical mammography, CT, and MRI, followed by histomorphological verification.

Main Results:

  • RIO demonstrated suitable physicochemical properties, with T1 and T2 relaxation times close to native Lipiodol.
  • The agent facilitated microangiography, 3D visualization, MI validation, and cause determination of death.
  • Digital radiography (DR) provided superior image quality for liver vasculature compared to MRI.

Conclusions:

  • Red iodized oil (RIO) is a multifunctional contrast dye with potential as an imaging biomarker.
  • It enables visualization of tissue vascularity, organ anatomy, and ex vivo validation.
  • RIO offers a versatile tool for preclinical research and diagnostic imaging.